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rsnap (0.1 in 2× saline-sodium phosphate-edta (sspe) buffer containing 6:1 tris(2-carboxyethyl)phosphine (tcep, pierce) to cys  (Thermo Fisher)


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    Thermo Fisher rsnap (0.1 in 2× saline-sodium phosphate-edta (sspe) buffer containing 6:1 tris(2-carboxyethyl)phosphine (tcep, pierce) to cys
    Rsnap (0.1 In 2× Saline Sodium Phosphate Edta (Sspe) Buffer Containing 6:1 Tris(2 Carboxyethyl)phosphine (Tcep, Pierce) To Cys, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/2+sspe+buffer/pmc02760437-52-44-54
    Average 90 stars, based on 1 article reviews
    rsnap (0.1 in 2× saline-sodium phosphate-edta (sspe) buffer containing 6:1 tris(2-carboxyethyl)phosphine (tcep, pierce) to cys - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Pathogen diversity of the neglected winter tick Alloceraea inermis in Hungary
    Article Snippet: .. The resulting PCR products from each tick were pooled individually in 2× SSPE buffer (Thermo Scientific, Vienna, Austria), which contained 0.3M NaCl, 0.02M NaH 2 PO 4 , 0.002M EDTA, and 0.1% sodium dodecyl sulfate (SDS, Applichem, Darmstadt, Germany). .. The resulting PCR products from each tick were pooled individually in 2× SSPE buffer (Thermo Scientific, Vienna, Austria), which contained 0.3M NaCl, 0.02M NaH 2 PO 4 , 0.002M EDTA, and 0.1% sodium dodecyl sulfate (SDS, Applichem, Darmstadt, Germany).

    Membrane:

    Article Title: Replication Kinetics for a Reporter Merkel Cell Polyomavirus
    Article Snippet: The DNA in the agarose gel was depurinated in 0.25 N HCl, treated with denaturation buffer (0.5 M NaOH, 1.5 M NaCl) and capillary-transferred onto a Hybond-N + Nylon Membrane (cat. no. RPN303B, GE Healthcare, Chicago, IL, USA) overnight with 10× SSC (1.5 M NaCl, 150 mM Sodium Citrate, pH 7.0). .. After UV crosslinking (Stratalinker, Stratagene, San Diego, CA, USA), the membrane was treated with prehybridization buffer (5× SSPE (diluted from 20× solution containing 3 M NaCl, 0.2 M sodium phosphate monobasic monohydrate, 0.2 M EDTA), 2% SDS, 1× Denhardts (diluted from 50× Denhardts, cat. no. 750018, Thermofisher, Waltham, MA, USA), 10% dextran sulfate, sonicated salmon sperm 10 μg/mL) for 4 h at 65 °C. .. A denatured, biotin-11dUTP-labeled MCV DNA probe generated with the Bio-prime Array CGH kit (cat. no. 45-0048, Invitrogen, Waltham, MA, USA) using a full-length MCV genome as template was then added to the prehybridization buffer and incubated overnight at 65 °C.

    Sonication:

    Article Title: Replication Kinetics for a Reporter Merkel Cell Polyomavirus
    Article Snippet: The DNA in the agarose gel was depurinated in 0.25 N HCl, treated with denaturation buffer (0.5 M NaOH, 1.5 M NaCl) and capillary-transferred onto a Hybond-N + Nylon Membrane (cat. no. RPN303B, GE Healthcare, Chicago, IL, USA) overnight with 10× SSC (1.5 M NaCl, 150 mM Sodium Citrate, pH 7.0). .. After UV crosslinking (Stratalinker, Stratagene, San Diego, CA, USA), the membrane was treated with prehybridization buffer (5× SSPE (diluted from 20× solution containing 3 M NaCl, 0.2 M sodium phosphate monobasic monohydrate, 0.2 M EDTA), 2% SDS, 1× Denhardts (diluted from 50× Denhardts, cat. no. 750018, Thermofisher, Waltham, MA, USA), 10% dextran sulfate, sonicated salmon sperm 10 μg/mL) for 4 h at 65 °C. .. A denatured, biotin-11dUTP-labeled MCV DNA probe generated with the Bio-prime Array CGH kit (cat. no. 45-0048, Invitrogen, Waltham, MA, USA) using a full-length MCV genome as template was then added to the prehybridization buffer and incubated overnight at 65 °C.

    Incubation:

    Article Title: Reproducible and sensitive micro-tissue RNA sequencing from formalin-fixed paraffin-embedded tissues for spatial gene expression analysis
    Article Snippet: For each sample, a proteinase K reaction solution containing 5 μL of PKD buffer (QIAGEN) and 0.31 μL of ProK (QIAGEN) was added and incubated for 1 h at 56 °C. .. The solution was then mixed thoroughly with vortexing, spun down, and incubated at 85 °C for 15 min. Oligo(dT) magnetic beads (Dynabeads Oligo (dT)25, 61002, Thermo Fisher) used for mRNA purification were pre-washed three times with equal volumes of 1× hybridization buffer (2× SSPE, 0.05% Tween20, and 0.0025% RNase inhibitor) and then suspended in half the volume of 2× hybridization buffer, in accordance with the instructions provided in the manual. ..

    Article Title: The double-stranded RNA-binding protein, Staufen1, is an IRES-transacting factor regulating HIV-1 cap-independent translation initiation.
    Article Snippet: For FISH/IF co-analyses (Figure 1A), a digoxigenin (DIG)-labeled RNA probe was synthesized in vitro in the presence of DIG-labeled UTP (Roche). .. For FISH probe hybridization to stain the vRNA, cells were DNase (Invitrogen) treated for 15 min (25 U per coverslip), then incubated in hybridization solution for 16–18 h at 42◦C (50% formamide, 1 mg/ml tRNA, 2× SSPE, 5× Denharts, 5 U RNaseOut (Invitrogen), 50 ng probe). ..

    Article Title: Reproducible and sensitive micro-tissue RNA-sequencing from formalin-fixed paraffin-embedded tissue for spatial gene expression analysis
    Article Snippet: For each sample, a proteinase K reaction solution containing 5 μL of PKD buffer (QIAGEN) and 0.31 μL of ProK (QIAGEN) was added and incubated for 1 h at 56 °C. .. The solution was then mixed thoroughly with vortexing, spun down, and incubated at 85 °C for 15 min. Oligo(dT) magnetic beads (Dynabeads Oligo (dT)25, 61002, Thermo Fisher) used for mRNA purification were pre-washed three times with equal volumes of 1× hybridization buffer (2× SSPE, 0.05% Tween20, and 0.0025% RNase inhibitor) and then suspended in half the volume of 2× hybridization buffer, in accordance with the instructions provided in the manual. ..

    Article Title: Argonaute-CLIP delineates versatile, functional RNAi networks in Aedes aegypti, a major vector of human viruses.
    Article Snippet: Resource Argonaute-CLIP delineates versatile, functional RNAi networks in Aedes aegypti, a major vector of human viruses Graphical abstract miRNA target miRNA pathway siRNA pathway A G O -C LI P target peaks miRNA loci (miRDeep2) AGO2 target preference target 1 bantam miR-1 miR-2-novel target 2 repression (imperfect, 3'UTRs) cleavage C on te xt In si gh t AGO2 modesTissue-specific target expression ovarian small RNAs ovary < all tissues target expression target 1 target 2 miR-11894novel esiRNA-1 miR-11895 CLIPflexR software package mosquito cell whole mosquitoOR R N A i n et w or k m ap s A na ly si s target peaks esiRNA loci (miRDeep2) esiRNA target transposons & endogenous viral elements 3'UTRs VS AGO2AGO1

    Magnetic Beads:

    Article Title: Reproducible and sensitive micro-tissue RNA sequencing from formalin-fixed paraffin-embedded tissues for spatial gene expression analysis
    Article Snippet: For each sample, a proteinase K reaction solution containing 5 μL of PKD buffer (QIAGEN) and 0.31 μL of ProK (QIAGEN) was added and incubated for 1 h at 56 °C. .. The solution was then mixed thoroughly with vortexing, spun down, and incubated at 85 °C for 15 min. Oligo(dT) magnetic beads (Dynabeads Oligo (dT)25, 61002, Thermo Fisher) used for mRNA purification were pre-washed three times with equal volumes of 1× hybridization buffer (2× SSPE, 0.05% Tween20, and 0.0025% RNase inhibitor) and then suspended in half the volume of 2× hybridization buffer, in accordance with the instructions provided in the manual. ..

    Article Title: Reproducible and sensitive micro-tissue RNA-sequencing from formalin-fixed paraffin-embedded tissue for spatial gene expression analysis
    Article Snippet: For each sample, a proteinase K reaction solution containing 5 μL of PKD buffer (QIAGEN) and 0.31 μL of ProK (QIAGEN) was added and incubated for 1 h at 56 °C. .. The solution was then mixed thoroughly with vortexing, spun down, and incubated at 85 °C for 15 min. Oligo(dT) magnetic beads (Dynabeads Oligo (dT)25, 61002, Thermo Fisher) used for mRNA purification were pre-washed three times with equal volumes of 1× hybridization buffer (2× SSPE, 0.05% Tween20, and 0.0025% RNase inhibitor) and then suspended in half the volume of 2× hybridization buffer, in accordance with the instructions provided in the manual. ..

    Purification:

    Article Title: Reproducible and sensitive micro-tissue RNA sequencing from formalin-fixed paraffin-embedded tissues for spatial gene expression analysis
    Article Snippet: For each sample, a proteinase K reaction solution containing 5 μL of PKD buffer (QIAGEN) and 0.31 μL of ProK (QIAGEN) was added and incubated for 1 h at 56 °C. .. The solution was then mixed thoroughly with vortexing, spun down, and incubated at 85 °C for 15 min. Oligo(dT) magnetic beads (Dynabeads Oligo (dT)25, 61002, Thermo Fisher) used for mRNA purification were pre-washed three times with equal volumes of 1× hybridization buffer (2× SSPE, 0.05% Tween20, and 0.0025% RNase inhibitor) and then suspended in half the volume of 2× hybridization buffer, in accordance with the instructions provided in the manual. ..

    Article Title: Reproducible and sensitive micro-tissue RNA-sequencing from formalin-fixed paraffin-embedded tissue for spatial gene expression analysis
    Article Snippet: For each sample, a proteinase K reaction solution containing 5 μL of PKD buffer (QIAGEN) and 0.31 μL of ProK (QIAGEN) was added and incubated for 1 h at 56 °C. .. The solution was then mixed thoroughly with vortexing, spun down, and incubated at 85 °C for 15 min. Oligo(dT) magnetic beads (Dynabeads Oligo (dT)25, 61002, Thermo Fisher) used for mRNA purification were pre-washed three times with equal volumes of 1× hybridization buffer (2× SSPE, 0.05% Tween20, and 0.0025% RNase inhibitor) and then suspended in half the volume of 2× hybridization buffer, in accordance with the instructions provided in the manual. ..

    Hybridization:

    Article Title: Reproducible and sensitive micro-tissue RNA sequencing from formalin-fixed paraffin-embedded tissues for spatial gene expression analysis
    Article Snippet: For each sample, a proteinase K reaction solution containing 5 μL of PKD buffer (QIAGEN) and 0.31 μL of ProK (QIAGEN) was added and incubated for 1 h at 56 °C. .. The solution was then mixed thoroughly with vortexing, spun down, and incubated at 85 °C for 15 min. Oligo(dT) magnetic beads (Dynabeads Oligo (dT)25, 61002, Thermo Fisher) used for mRNA purification were pre-washed three times with equal volumes of 1× hybridization buffer (2× SSPE, 0.05% Tween20, and 0.0025% RNase inhibitor) and then suspended in half the volume of 2× hybridization buffer, in accordance with the instructions provided in the manual. ..

    Article Title: The double-stranded RNA-binding protein, Staufen1, is an IRES-transacting factor regulating HIV-1 cap-independent translation initiation.
    Article Snippet: For FISH/IF co-analyses (Figure 1A), a digoxigenin (DIG)-labeled RNA probe was synthesized in vitro in the presence of DIG-labeled UTP (Roche). .. For FISH probe hybridization to stain the vRNA, cells were DNase (Invitrogen) treated for 15 min (25 U per coverslip), then incubated in hybridization solution for 16–18 h at 42◦C (50% formamide, 1 mg/ml tRNA, 2× SSPE, 5× Denharts, 5 U RNaseOut (Invitrogen), 50 ng probe). ..

    Article Title: Reproducible and sensitive micro-tissue RNA-sequencing from formalin-fixed paraffin-embedded tissue for spatial gene expression analysis
    Article Snippet: For each sample, a proteinase K reaction solution containing 5 μL of PKD buffer (QIAGEN) and 0.31 μL of ProK (QIAGEN) was added and incubated for 1 h at 56 °C. .. The solution was then mixed thoroughly with vortexing, spun down, and incubated at 85 °C for 15 min. Oligo(dT) magnetic beads (Dynabeads Oligo (dT)25, 61002, Thermo Fisher) used for mRNA purification were pre-washed three times with equal volumes of 1× hybridization buffer (2× SSPE, 0.05% Tween20, and 0.0025% RNase inhibitor) and then suspended in half the volume of 2× hybridization buffer, in accordance with the instructions provided in the manual. ..

    Article Title: Enrichment of low abundance DNA/RNA by oligonucleotide-clicked iron oxide nanoparticles
    Article Snippet: .. Capture probe mix was prepared by mixing the 500 ng biotin-labeled probe set (pool of eight 5'-biotin-labeled probes at equal concentrations) with 20U RNase Block Ribonuclease Inhibitor and ultra-pure water to a final volume of 7 μL, pre-warmed to 65 °C for at least 2 min. Hybridization buffer (10X SSPE, 10X Denhardt's solution, 10 mM EDTA, 0.2% SDS and 20U RNase block) was freshly prepared and pre-warmed at 65 °C for at least 5 min. 13 μL hybridization buffer and 7 μL capture probe mix were rapidly added to the pre-capture library mix to make the hybridization mix in a final volume of 29 μL, and kept at 65 °C for 24 h. 50 μL of Dynabeads MyOne streptavidin T1 beads (Thermo Fisher Scientific) were washed twice with 200 μL binding buffer and re-suspended with 200 μL binding buffer. ..

    Article Title: Wheat stem rust resistance genes and methods of use
    Article Snippet: DNA libraries were enriched according to the MYbaits (MYcroarray) protocol and using MYbaits reagents. .. Briefly, 500 ng of the prepped libraries were hybridized in hybridization buffer (10×SSPE, 10×Denhardt's solution, 10 mM EDTA, 0.2% SDS) to the biotinylated RNA baits for 40 h at 65° C. After hybridization, bound DNA was recovered using magnetic streptavidin-coated beads as follows: the hybridization mix was added to Dynabeads MyOne Streptavidin C1 (Invitrogen, Life Technologies) that had been washed three times and resuspended in binding buffer (1 M NaCl; 10 mM Tris-HCl, pH 7.5; 1 mM EDTA). ..

    Fluorescence In Situ Hybridization:

    Article Title: The double-stranded RNA-binding protein, Staufen1, is an IRES-transacting factor regulating HIV-1 cap-independent translation initiation.
    Article Snippet: For FISH/IF co-analyses (Figure 1A), a digoxigenin (DIG)-labeled RNA probe was synthesized in vitro in the presence of DIG-labeled UTP (Roche). .. For FISH probe hybridization to stain the vRNA, cells were DNase (Invitrogen) treated for 15 min (25 U per coverslip), then incubated in hybridization solution for 16–18 h at 42◦C (50% formamide, 1 mg/ml tRNA, 2× SSPE, 5× Denharts, 5 U RNaseOut (Invitrogen), 50 ng probe). ..

    Staining:

    Article Title: The double-stranded RNA-binding protein, Staufen1, is an IRES-transacting factor regulating HIV-1 cap-independent translation initiation.
    Article Snippet: For FISH/IF co-analyses (Figure 1A), a digoxigenin (DIG)-labeled RNA probe was synthesized in vitro in the presence of DIG-labeled UTP (Roche). .. For FISH probe hybridization to stain the vRNA, cells were DNase (Invitrogen) treated for 15 min (25 U per coverslip), then incubated in hybridization solution for 16–18 h at 42◦C (50% formamide, 1 mg/ml tRNA, 2× SSPE, 5× Denharts, 5 U RNaseOut (Invitrogen), 50 ng probe). ..

    Blocking Assay:

    Article Title: Enrichment of low abundance DNA/RNA by oligonucleotide-clicked iron oxide nanoparticles
    Article Snippet: .. Capture probe mix was prepared by mixing the 500 ng biotin-labeled probe set (pool of eight 5'-biotin-labeled probes at equal concentrations) with 20U RNase Block Ribonuclease Inhibitor and ultra-pure water to a final volume of 7 μL, pre-warmed to 65 °C for at least 2 min. Hybridization buffer (10X SSPE, 10X Denhardt's solution, 10 mM EDTA, 0.2% SDS and 20U RNase block) was freshly prepared and pre-warmed at 65 °C for at least 5 min. 13 μL hybridization buffer and 7 μL capture probe mix were rapidly added to the pre-capture library mix to make the hybridization mix in a final volume of 29 μL, and kept at 65 °C for 24 h. 50 μL of Dynabeads MyOne streptavidin T1 beads (Thermo Fisher Scientific) were washed twice with 200 μL binding buffer and re-suspended with 200 μL binding buffer. ..

    Binding Assay:

    Article Title: Enrichment of low abundance DNA/RNA by oligonucleotide-clicked iron oxide nanoparticles
    Article Snippet: .. Capture probe mix was prepared by mixing the 500 ng biotin-labeled probe set (pool of eight 5'-biotin-labeled probes at equal concentrations) with 20U RNase Block Ribonuclease Inhibitor and ultra-pure water to a final volume of 7 μL, pre-warmed to 65 °C for at least 2 min. Hybridization buffer (10X SSPE, 10X Denhardt's solution, 10 mM EDTA, 0.2% SDS and 20U RNase block) was freshly prepared and pre-warmed at 65 °C for at least 5 min. 13 μL hybridization buffer and 7 μL capture probe mix were rapidly added to the pre-capture library mix to make the hybridization mix in a final volume of 29 μL, and kept at 65 °C for 24 h. 50 μL of Dynabeads MyOne streptavidin T1 beads (Thermo Fisher Scientific) were washed twice with 200 μL binding buffer and re-suspended with 200 μL binding buffer. ..

    Article Title: Argonaute-CLIP delineates versatile, functional RNAi networks in Aedes aegypti, a major vector of human viruses.
    Article Snippet: Resource Argonaute-CLIP delineates versatile, functional RNAi networks in Aedes aegypti, a major vector of human viruses Graphical abstract miRNA target miRNA pathway siRNA pathway A G O -C LI P target peaks miRNA loci (miRDeep2) AGO2 target preference target 1 bantam miR-1 miR-2-novel target 2 repression (imperfect, 3'UTRs) cleavage C on te xt In si gh t AGO2 modesTissue-specific target expression ovarian small RNAs ovary < all tissues target expression target 1 target 2 miR-11894novel esiRNA-1 miR-11895 CLIPflexR software package mosquito cell whole mosquitoOR R N A i n et w or k m ap s A na ly si s target peaks esiRNA loci (miRDeep2) esiRNA target transposons & endogenous viral elements 3'UTRs VS AGO2AGO1

    Article Title: Wheat stem rust resistance genes and methods of use
    Article Snippet: DNA libraries were enriched according to the MYbaits (MYcroarray) protocol and using MYbaits reagents. .. Briefly, 500 ng of the prepped libraries were hybridized in hybridization buffer (10×SSPE, 10×Denhardt's solution, 10 mM EDTA, 0.2% SDS) to the biotinylated RNA baits for 40 h at 65° C. After hybridization, bound DNA was recovered using magnetic streptavidin-coated beads as follows: the hybridization mix was added to Dynabeads MyOne Streptavidin C1 (Invitrogen, Life Technologies) that had been washed three times and resuspended in binding buffer (1 M NaCl; 10 mM Tris-HCl, pH 7.5; 1 mM EDTA). ..



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